Anti-human Interleukin(IL)-4 Clone 8D4-8 Cross-Reacts With Myosin-9 Associated With Apoptotic Cells and Should Not Be Used for Flow Cytometry Applications Querying IL-4 Expression

Anti-human Interleukin(IL)-4 Clone 8D4-8 Cross-Reacts With Myosin-9 Associated With Apoptotic Cells and Should Not Be Used for Flow Cytometry Applications Querying IL-4 Expression

Interleukin(IL)-4 is produced by T cells and other leukocytes and is a critical mediator of monocyte and B cell responses. During routine flow cytometry panel validation for the investigation of intracellular cytokines, we observed unique IL-4 expression patterns associated with the widely available...

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Bibliographic Details
Main Authors: Robert Z. Harms, Kiana Borengasser, Vikas Kumar, Nora Sarvetnick
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-04-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
interleukin-4
myosin-9
cross-reacting antibodies
8D4-8
apoptosis
flow cytometry
Online Access:https://www.frontiersin.org/article/10.3389/fcell.2019.00046/full

Internet

https://www.frontiersin.org/article/10.3389/fcell.2019.00046/full

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