Structural basis for the promiscuous PAM recognition by Corynebacterium diphtheriae Cas9

Structural basis for the promiscuous PAM recognition by Corynebacterium diphtheriae Cas9

The RNA-guided DNA endonuclease Cas9 from Corynebacterium diphtheriae (CdCas9) recognizes a promiscuous protospacer adjacent motif (PAM). Here the authors provide insights into the CdCas9-mediated PAM recognition mechanism by determining the 2.9 Å crystal structure of CdCas9 in complex with the guid...

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Bibliographic Details
Main Authors: Seiichi Hirano, Omar O. Abudayyeh, Jonathan S. Gootenberg, Takuro Horii, Ryuichiro Ishitani, Izuho Hatada, Feng Zhang, Hiroshi Nishimasu, Osamu Nureki
Format: Article
Language:English
Published: Nature Publishing Group 2019-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-09741-6

Internet

https://doi.org/10.1038/s41467-019-09741-6

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