Development of a multiplex qPCR-based approach for the diagnosis of Dirofilaria immitis, D. repens and Acanthocheilonema reconditum
Abstract Background Dirofilaria immitis, D. repens and Acanthocheilonema reconditum are the main causative agents of zoonotic canine filariosis. Methods We developed a combined multiplex approach for filaria and Wolbachia detection using the 28S-based pan-filarial and 16S-based pan-Wolbachia qPCRs,...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2020-06-01
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Series: | Parasites & Vectors |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s13071-020-04185-0 |