Quantification of polysaccharides fixed to Gram stained slides using lactophenol cotton blue and digital image processing [version 5; referees: 2 approved, 1 approved with reservations]

Dark blue rings and circles emerged when the non-specific polysaccharide stain lactophenol cotton blue was added to Gram stained slides. The dark blue staining is attributable to the presence of capsular polysaccharides and bacterial slime associated with clumps of Gram-negative bacteria.  Since all...

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Bibliographic Details
Main Author: Bryan Ericksen
Format: Article
Language:English
Published: F1000 Research Ltd 2017-12-01
Series:F1000Research
Subjects:
Online Access:https://f1000research.com/articles/4-1/v5