Quantification of 3-ketodihydrosphingosine using HPLC-ESI-MS/MS to study SPT activity in yeast Saccharomyces cerevisiae[S]

Quantification of 3-ketodihydrosphingosine using HPLC-ESI-MS/MS to study SPT activity in yeast Saccharomyces cerevisiae[S]

Serine palmitoyltransferase (SPT) catalyzes the rate-limiting step of condensation of L-serine and palmitoyl-CoA to form 3-ketodihydrosphingosine (3KDS). Here, we report a HPLC-ESI-MS/MS method to directly quantify 3KDS generated by SPT. With this technique, we were able to detect 3KDS at a level co...

Full description

Bibliographic Details
Main Authors: Jihui Ren, Justin Snider, Michael V. Airola, Aaron Zhong, Nadia A. Rana, Lina M. Obeid, Yusuf A. Hannun
Format: Article
Language:English
Published: Elsevier 2018-01-01
Series:Journal of Lipid Research
Subjects:
serine palmitoyltransferase
high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry
sphingolipids
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520339420

Internet

http://www.sciencedirect.com/science/article/pii/S0022227520339420

Similar Items