Site‐directed mutagenesis, in vivo electroporation and mass spectrometry in search for determinants of the subcellular targeting of Rab7b paralogue in the model eukaryote Paramecium octaurelia

Site‐directed mutagenesis, in vivo electroporation and mass spectrometry in search for determinants of the subcellular targeting of Rab7b paralogue in the model eukaryote Paramecium octaurelia

<p>Protein products of the paralogous genes resulting from the whole genome duplication may acquire new function. The role of post‐translational modifications (PTM) in proper targeting of <em>Paramecium</em> Rab7b paralogue – distinct from that of Rab7a directly involved in phagocy...

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Bibliographic Details
Main Authors: E. Wyroba, P. Kwaśniak, K. Miller, K. Kobyłecki, M. Osińska
Format: Article
Language:English
Published: PAGEPress Publications 2016-04-01
Series:European Journal of Histochemistry
Subjects:
Point mutagenesis
electroporation
post-translational modifications
mass spectrometry
glycosylation
Rab7
Paramecium
recombinant proteins.
Online Access:http://www.ejh.it/index.php/ejh/article/view/2612

Internet

http://www.ejh.it/index.php/ejh/article/view/2612

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