A new reverse transcription-polymerase chain reaction method for accurate quantification

<p>Abstract</p> <p>Background</p> <p>Reverse transcription-polymerase chain reaction (RT-PCR) is a very sensitive technique to measure and to compare mRNA levels among samples. However, it is extremely difficult to maintain linearity across the entire procedure, especia...

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Bibliographic Details
Main Author: Shiao Yih-Horng
Format: Article
Language:English
Published: BMC 2003-12-01
Series:BMC Biotechnology
Online Access:http://www.biomedcentral.com/1472-6750/3/22