Use of the heteroduplex mobility assay and cell sorting to select genome sequences of the CCR5 gene in HEK 293T cells edited by transcription activator-like effector nucleases

Use of the heteroduplex mobility assay and cell sorting to select genome sequences of the CCR5 gene in HEK 293T cells edited by transcription activator-like effector nucleases

Engineered nucleases such as zinc finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN) are one of the most promising tools for modifying genomes. These site-specific enzymes cause double- strand breaks that allow gene disruption or gene insertion, thereby facilitating g...

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Bibliographic Details
Main Authors: Arildo Nerys-Junior, Lendel C. Costa, Luciene P. Braga-Dias, Márcia Oliveira, Átila D. Rossi, Rodrigo Delvecchio da Cunha, Gabriel S. Gonçalves, Amilcar Tanuri
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2014-01-01
Series:Genetics and Molecular Biology
Subjects:
CCR5
genome editing
gene knockout
TALEN
HMA
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100018&lng=en&tlng=en

Internet

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100018&lng=en&tlng=en

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