Quantitative Microdialysis: Experimental Protocol and Software for Small Molecule Protein Affinity Determination and for Exclusion of Compounds with Poor Physicochemical Properties

Quantitative Microdialysis: Experimental Protocol and Software for Small Molecule Protein Affinity Determination and for Exclusion of Compounds with Poor Physicochemical Properties

Quantitative microdialysis is a traditional biophysical affinity determination technique. In the development of the detailed experimental protocol presented, we used commercially available equipment, rapid equilibrium dialysis (RED) devices (ThermoFisher Scientific), which means that it is open to m...

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Bibliographic Details
Main Authors: Steven Shave, Nhan T. Pham, Connor B. Śmieja, Manfred Auer
Format: Article
Language:English
Published: MDPI AG 2020-07-01
Series:Methods and Protocols
Subjects:
label free screening
affinity determination
dialysis
K<sub>D</sub> determination
promiscuity
aggregation
Online Access:https://www.mdpi.com/2409-9279/3/3/55

Internet

https://www.mdpi.com/2409-9279/3/3/55

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