Recombinant expression and characterization of the cytoplasmic rice β-glucosidase Os1BGlu4.

The Os1BGlu4 β-glucosidase is the only glycoside hydrolase family 1 member in rice that is predicted to be localized in the cytoplasm. To characterize the biochemical function of rice Os1BGlu4, the Os1bglu4 cDNA was cloned and used to express a thioredoxin fusion protein in Escherichia coli. After r...

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Bibliographic Details
Main Authors: Chen Rouyi, Supaporn Baiya, Sang-Kyu Lee, Bancha Mahong, Jong-Seong Jeon, James R Ketudat-Cairns, Mariena Ketudat-Cairns
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24802508/pdf/?tool=EBI