In Vivo Microdialysis of Endogenous and ¹³C-labeled TCA Metabolites in Rat Brain: Reversible and Persistent Effects of Mitochondrial Inhibition and Transient Cerebral Ischemia

• Main Authors: Jesper F. Havelund, Kevin H. Nygaard, Troels H. Nielsen, Carl-Henrik Nordström, Frantz R. Poulsen, Nils. J. Færgeman, Axel Forsse, Jan Bert Gramsbergen
• Format: Article
• Language: English
• Published: MDPI AG 2019-09-01
• Series: Metabolites
• Subjects: ¹³c-labeled succinate; cerebral ischemia; energy metabolism; endothelin-1; lc-ms; malonate; micro-dialysis; mitochondrial dysfunction; reperfusion; tricarboxylic acid cycle
• Online Access: https://www.mdpi.com/2218-1989/9/10/204

Cerebral micro-dialysis allows continuous sampling of extracellular metabolites, including glucose, lactate and pyruvate. Transient ischemic events cause a rapid drop in glucose and a rise in lactate levels. Following such events, the lactate/pyruvate (L/P) ratio may remain elevated for a prolonged period of time. In neurointensive care clinics, this ratio is considered a metabolic marker of ischemia and/or mitochondrial dysfunction. Here we propose a novel, sensitive microdialysis liquid chromatography-mass spectrometry (LC-MS) approach to monitor mitochondrial dysfunction in living brain using perfusion with ¹³C-labeled succinate and analysis of ¹³C-labeled tricarboxylic acid cycle (TCA) intermediates. This approach was evaluated in rat brain using malonate-perfusion (10−50 mM) and endothelin-1 (ET-1)-induced transient cerebral ischemia. In the malonate model, the expected changes upon inhibition of succinate dehydrogenase (SDH) were observed, i.e., an increase in endogenous succinate and decreases in fumaric acid and malic acid. The inhibition was further elaborated by incorporation of ¹³C into specific TCA intermediates from ¹³C-labeled succinate. In the ET-1 model, increases in non-labeled TCA metabolites (reflecting release of intracellular compounds) and decreases in ¹³C-labeled TCA metabolites (reflecting inhibition of de novo synthesis) were observed. The analysis of ¹³C incorporation provides further layers of information to identify metabolic disturbances in experimental models and neuro-intensive care patients.