A CRISPR Interference System for Efficient and Rapid Gene Knockdown in Caulobacter crescentus
Caulobacter crescentus is a major model organism for understanding cell cycle regulation and cellular asymmetry. The current genetic tools for deleting or silencing the expression of individual genes, particularly those essential for viability, are time-consuming and labor-intensive, which limits gl...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2020-01-01
|
| Series: | mBio |
| Subjects: | |
| Online Access: | https://doi.org/10.1128/mBio.02415-19 |