Purification of Islets of Langerhans from Porcine Pancreas

Purification of Islets of Langerhans from Porcine Pancreas

Flotation through a slightly hyperosmotic discontinuous gradient iodixanol achieves a much higher recovery of islets of an improved viability than the customary method using sedimentation on to a diatrizoate/polysaccharide barrier. Flotation techniques achieve an enhanced separation of the islets fr...

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Main Author: John Graham
Format: Article
Language:English
Published: Hindawi Limited 2002-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/tsw.2002.847
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spelling doaj-567c25536914403a94d2da1603d349902020-11-25T00:54:23ZengHindawi LimitedThe Scientific World Journal1537-744X2002-01-0121657166110.1100/tsw.2002.847Purification of Islets of Langerhans from Porcine PancreasJohn Graham0School of Biomolecular Sciences, Liverpool John Moores University, UKFlotation through a slightly hyperosmotic discontinuous gradient iodixanol achieves a much higher recovery of islets of an improved viability than the customary method using sedimentation on to a diatrizoate/polysaccharide barrier. Flotation techniques achieve an enhanced separation of the islets from any residual digestive enzymes and from acinar cells. The method has been extended to human pancreas.http://dx.doi.org/10.1100/tsw.2002.847
collection DOAJ
language English
format Article
sources DOAJ
author John Graham
spellingShingle John Graham
Purification of Islets of Langerhans from Porcine Pancreas
The Scientific World Journal
author_facet John Graham
author_sort John Graham
title Purification of Islets of Langerhans from Porcine Pancreas
title_short Purification of Islets of Langerhans from Porcine Pancreas
title_full Purification of Islets of Langerhans from Porcine Pancreas
title_fullStr Purification of Islets of Langerhans from Porcine Pancreas
title_full_unstemmed Purification of Islets of Langerhans from Porcine Pancreas
title_sort purification of islets of langerhans from porcine pancreas
publisher Hindawi Limited
series The Scientific World Journal
issn 1537-744X
publishDate 2002-01-01
description Flotation through a slightly hyperosmotic discontinuous gradient iodixanol achieves a much higher recovery of islets of an improved viability than the customary method using sedimentation on to a diatrizoate/polysaccharide barrier. Flotation techniques achieve an enhanced separation of the islets from any residual digestive enzymes and from acinar cells. The method has been extended to human pancreas.
url http://dx.doi.org/10.1100/tsw.2002.847
work_keys_str_mv AT johngraham purificationofisletsoflangerhansfromporcinepancreas
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