Purification of Islets of Langerhans from Porcine Pancreas

Flotation through a slightly hyperosmotic discontinuous gradient iodixanol achieves a much higher recovery of islets of an improved viability than the customary method using sedimentation on to a diatrizoate/polysaccharide barrier. Flotation techniques achieve an enhanced separation of the islets fr...

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Bibliographic Details
Main Author: John Graham
Format: Article
Language:English
Published: Hindawi Limited 2002-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/tsw.2002.847