Enhancing PCR Amplification of DNA from Recalcitrant Plant Specimens Using a Trehalose-Based Additive

Premise of the study: PCR amplification of DNA extracted from plants is sometimes difficult due to the presence of inhibitory compounds. An effective method to overcome the inhibitory effect of compounds that contaminate DNA from difficult plant specimens is needed. Methods and Results: The effec...

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Bibliographic Details
Main Authors: Tharangamala Samarakoon, Shiao Y. Wang, Mac H. Alford
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:Applications in Plant Sciences
Subjects:
DNA
PCR
Online Access:http://www.bioone.org/doi/full/10.3732/apps.1200236