Mutational optimization of the coelenterazine-dependent luciferase from <it>Renilla</it>
<p>Abstract</p> <p><it>Renilla </it>luciferase (RLUC) is a popular reporter enzyme for gene expression and biosensor applications, but it is an unstable enzyme whose catalytic mechanism remains to be elucidated. We titrated that one RLUC molecule can turn over about one...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2008-09-01
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| Series: | Plant Methods |
| Online Access: | http://www.plantmethods.com/content/4/1/23 |