Construction and Analysis of Suppression Subtractive cDNA Library from Fertile Disk Floret Buds of Zinnia elegans

Construction and Analysis of Suppression Subtractive cDNA Library from Fertile Disk Floret Buds of Zinnia elegans

In order to isolate genes related to anther development and understand molecular basis of male sterility, cDNA library of Zinnia elegans was constructed using suppression subtractive hybridization (SSH) approach. 672 different expressed clones were selected from the fertile disk floret buds. PCR res...

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Bibliographic Details
Main Authors: Rui-hua PANG, Guang-ying MA, Xue-yuan LOU, Man-zhu BAO, Yao-mei YE
Format: Article
Language:English
Published: Elsevier 2012-04-01
Series:Journal of Integrative Agriculture
Subjects:
Zinnia elegans
suppression subtractive hybridization (SSH)
male sterility
anther development
expressed sequence tags (ESTs)
Online Access:http://www.sciencedirect.com/science/article/pii/S2095311912600434

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http://www.sciencedirect.com/science/article/pii/S2095311912600434

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