Development of an NT-ProBNP Assay Reagent Based on High Specific Activity Alkaline Phosphatase CmAP and an Improved Coupling Method

(1) Background: Chemiluminescent enzyme immunoassay (CLEIA) is an efficient analytical method. Alkaline phosphatase (ALP) with high specific activity is the basis for CLEIA to achieve high sensitivity. In this study, a high specific activity <i>Cobetia marina</i> ALP (CmAP) and an improv...

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Bibliographic Details
Main Authors: Hai-Chao Li, Xin He, Shan-Peng Qiao, Zhen-Ni Liu, Yu-Zhou Gao
Format: Article
Language:English
Published: MDPI AG 2020-12-01
Series:Applied Sciences
Subjects:
Online Access:https://www.mdpi.com/2076-3417/10/23/8682