A novel rapid and reproducible flow cytometric method for optimization of transfection efficiency in cells.

Transfection is one of the most frequently used techniques in molecular biology that is also applicable for gene therapy studies in humans. One of the biggest challenges to investigate the protein function and interaction in gene therapy studies is to have reliable monospecific detection reagents, p...

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Main Authors: Stefanie Homann, Christian Hofmann, Aleksandr M Gorin, Huy Cong Xuan Nguyen, Diana Huynh, Phillip Hamid, Neil Maithel, Vahe Yacoubian, Wenli Mu, Athanasios Kossyvakis, Shubhendu Sen Roy, Otto Orlean Yang, Theodoros Kelesidis
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5580984?pdf=render