Cloning and Expression of <i>ace</i> Gene of <i>Vibrio cholerae</i> in <i>Escherichia coli</i>
Accessory cholera enterotoxin (ace) gene of Vibrio cholerae, amplified by PCR, is cloned into the BamHI-HindIII sites using plasmid pQE30. Expression of ace gene is under the control of T5 promotor. The Escherichia coli strain M15[pREP4]pAce90 (KM 194), containing recombinant plasmid, is an active p...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2012-04-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/792 |