Prolonging culture of primary human keratinocytes isolated from suction blisters with the Rho kinase inhibitor Y-27632.

Keratinocytes are the most abundant cell type in the epidermis. They prevent desiccation and provide immunological and barrier defense against potential pathogens such as Staphylococcus aureus and Candida albicans. The study of this first line of immune defense may be hindered by invasive isolation...

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Bibliographic Details
Main Authors: Erik D Anderson, Inka Sastalla, Noah J Earland, Minai Mahnaz, Ian N Moore, Francisco Otaizo-Carrasquero, Timothy G Myers, Christopher A Myles, Sandip K Datta, Ian A Myles
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC6135349?pdf=render