Soluble expression of a novel feruloyl esterase from Burkholderia pyrrocinia B1213 in Escherichia coli and optimization of production conditions

This report examines the soluble expression of a novel feruloyl esterase, BpFae, from Burkholderia pyrrocinia in Escherichia coli overexpression systems using three expression vectors, pET28a, pCold-TF and pGEX-4T-1. BpFae was overexpressed as insoluble inclusion bodies when pET28a was used as the e...

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Bibliographic Details
Main Authors: Zhilei Fu, Guangsen Fan, Yuting Zhu, Chao Teng, Hehe Li, Qian Liu, Ran Yang, Xiuting Li
Format: Article
Language:English
Published: Taylor & Francis Group 2020-01-01
Series:Biotechnology & Biotechnological Equipment
Subjects:
Online Access:http://dx.doi.org/10.1080/13102818.2020.1803129