Highly Efficient Protoplast Isolation and Transient Expression System for Functional Characterization of Flowering Related Genes in <i>Cymbidium</i> Orchids

Highly Efficient Protoplast Isolation and Transient Expression System for Functional Characterization of Flowering Related Genes in <i>Cymbidium</i> Orchids

Protoplast systems have been proven powerful tools in modern plant biology. However, successful preparation of abundant viable protoplasts remains a challenge for <i>Cymbidium</i> orchids. Herein, we established an efficient protoplast isolation protocol from orchid petals through optimi...

Full description

Bibliographic Details
Main Authors: Rui Ren, Jie Gao, Chuqiao Lu, Yonglu Wei, Jianpeng Jin, Sek-Man Wong, Genfa Zhu, Fengxi Yang
Format: Article
Language:English
Published: MDPI AG 2020-03-01
Series:International Journal of Molecular Sciences
Subjects:
cymbidium orchids, protoplast isolation, protoplast-based transient expression system (ptes), subcellular location, bimolecular fluorescence complementation (bifc) assay, gene regulation
Online Access:https://www.mdpi.com/1422-0067/21/7/2264

Internet

https://www.mdpi.com/1422-0067/21/7/2264

Similar Items