Modulating chromatin accessibility by transactivation and targeting proximal dsgRNAs enhances Cas9 editing efficiency in vivo

Modulating chromatin accessibility by transactivation and targeting proximal dsgRNAs enhances Cas9 editing efficiency in vivo

Abstract The CRISPR/Cas9 system is unable to edit all targetable genomic sites with full efficiency in vivo. We show that Cas9-mediated editing is more efficient in open chromatin regions than in closed chromatin regions in rice. A construct (Cas9-TV) formed by fusing a synthetic transcription activ...

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Bibliographic Details
Main Authors: Guanwen Liu, Kangquan Yin, Qianwei Zhang, Caixia Gao, Jin-Long Qiu
Format: Article
Language:English
Published: BMC 2019-07-01
Series:Genome Biology
Subjects:
CRISPR/Cas9
Chromatin accessibility
Transcription activation domain
Proximal dsgRNA
Online Access:http://link.springer.com/article/10.1186/s13059-019-1762-8

Internet

http://link.springer.com/article/10.1186/s13059-019-1762-8

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