Methylase-assisted subcloning for high throughput BioBrick assembly

The BioBrick standard makes possible iterated pairwise assembly of cloned parts without any depletion of unique restriction sites. Every part that conforms to the standard is compatible with every other part, thereby fostering a worldwide user community. The assembly methods, however, are labor inte...

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Bibliographic Details
Main Author: Ichiro Matsumura
Format: Article
Language:English
Published: PeerJ Inc. 2020-09-01
Series:PeerJ
Subjects:
Online Access:https://peerj.com/articles/9841.pdf