Methylase-assisted subcloning for high throughput BioBrick assembly
The BioBrick standard makes possible iterated pairwise assembly of cloned parts without any depletion of unique restriction sites. Every part that conforms to the standard is compatible with every other part, thereby fostering a worldwide user community. The assembly methods, however, are labor inte...
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Format: | Article |
Language: | English |
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PeerJ Inc.
2020-09-01
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Series: | PeerJ |
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Online Access: | https://peerj.com/articles/9841.pdf |