CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome

CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome

CRISPR/Cas9 gene editing has been used to generate mutations in several mouse genes. Here, the authors show that targeting events using single guide RNAs cause large deletions at 17 sites in the mouse genome, suggesting that careful genotyping is needed and sequential targeting may avoid such deleti...

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Bibliographic Details
Main Authors: Ha Youn Shin, Chaochen Wang, Hye Kyung Lee, Kyung Hyun Yoo, Xianke Zeng, Tyler Kuhns, Chul Min Yang, Teresa Mohr, Chengyu Liu, Lothar Hennighausen
Format: Article
Language:English
Published: Nature Publishing Group 2017-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/ncomms15464

Internet

https://doi.org/10.1038/ncomms15464

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