A Mass Spectrometry-Based Approach for Mapping Protein Subcellular Localization Reveals the Spatial Proteome of Mouse Primary Neurons

A Mass Spectrometry-Based Approach for Mapping Protein Subcellular Localization Reveals the Spatial Proteome of Mouse Primary Neurons

We previously developed a mass spectrometry-based method, dynamic organellar maps, for the determination of protein subcellular localization and identification of translocation events in comparative experiments. The use of metabolic labeling for quantification (stable isotope labeling by amino acids...

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Bibliographic Details
Main Authors: Daniel N. Itzhak, Colin Davies, Stefka Tyanova, Archana Mishra, James Williamson, Robin Antrobus, Jürgen Cox, Michael P. Weekes, Georg H.H. Borner
Format: Article
Language:English
Published: Elsevier 2017-09-01
Series:Cell Reports
Subjects:
spatial proteomics
organellar proteomics
neurons
primary cells
quantitative mass spectrometry
label-free quantification
tandem mass tagging
TMT
LFQ
EGF signaling
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124717311889

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http://www.sciencedirect.com/science/article/pii/S2211124717311889

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