Measurement of mitochondrial respiration in adherent cells by Seahorse XF96 Cell Mito Stress Test

Summary: Mitochondria play pivotal roles in cellular energy metabolism. Most of the intracellular adenosine triphosphate (ATP) is generated by mitochondrial respiration. The Cell Mito Stress Test is a common method to measure the key parameters of mitochondrial respiration. Here, we use the human ce...

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Bibliographic Details
Main Authors: Xia Gu, Yuan Ma, Yi Liu, Qiang Wan
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672030232X