Efficient CRISPR/Cas9 mediated Pooled-sgRNAs assembly accelerates targeting multiple genes related to male sterility in cotton

Efficient CRISPR/Cas9 mediated Pooled-sgRNAs assembly accelerates targeting multiple genes related to male sterility in cotton

Abstract Background Upland cotton (Gossypium hirsutum), harboring a complex allotetraploid genome, consists of A and D sub-genomes. Every gene has multiple copies with high sequence similarity that makes genetic, genomic and functional analyses extremely challenging. The recent accessibility of CRIS...

Full description

Bibliographic Details
Main Authors: Mohamed Ramadan, Muna Alariqi, Yizan Ma, Yanlong Li, Zhenping Liu, Rui Zhang, Shuangxia Jin, Ling Min, Xianlong Zhang
Format: Article
Language:English
Published: BMC 2021-02-01
Series:Plant Methods
Subjects:
Cotton
CRISPR/Cas9
Genome editing
Male sterility
Pooled sgRNAs assembly
Online Access:https://doi.org/10.1186/s13007-021-00712-x

Internet

https://doi.org/10.1186/s13007-021-00712-x

Similar Items