A two-hybrid antibody micropattern assay reveals specific in cis interactions of MHC I heavy chains at the cell surface

A two-hybrid antibody micropattern assay reveals specific in cis interactions of MHC I heavy chains at the cell surface

We demonstrate a two-hybrid assay based on antibody micropatterns to study protein-protein interactions at the cell surface of major histocompatibility complex class I (MHC I) proteins. Anti-tag and conformation-specific antibodies are used for individual capture of specific forms of MHC I proteins...

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Bibliographic Details
Main Authors: Cindy Dirscherl, Zeynep Hein, Venkat Raman Ramnarayan, Catherine Jacob-Dolan, Sebastian Springer
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2018-09-01
Series:eLife
Subjects:
major histocompatibility complex molecules
protein micropatterns
in cis interactions
endocytosis
membrane proteins
Online Access:https://elifesciences.org/articles/34150

Internet

https://elifesciences.org/articles/34150

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