Protocol for the generation of murine bronchiolospheres

Summary: Organoid models have been shown to be valuable tools for studying epithelial-mesenchymal crosstalk during biological and pathological settings. Our data identified ACTA2+ PDGFRα+ repair-supportive mesenchymal cells as an important component of the conducting airway niche. Here, we provide a...

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Bibliographic Details
Main Authors: Ana Ivonne Vazquez-Armendariz, Werner Seeger, Susanne Herold, Elie El Agha
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721003014