XACT-seq: A photocrosslinking-based technique for detection of the RNA polymerase active-center position relative to DNA in Escherichia coli

XACT-seq: A photocrosslinking-based technique for detection of the RNA polymerase active-center position relative to DNA in Escherichia coli

Summary: XACT-seq (“crosslink between active-center and template sequencing”) is a technique for high-throughput, single-nucleotide resolution mapping of RNA polymerase (RNAP) active-center positions relative to the DNA template. XACT-seq overcomes limitations of approaches that rely on analysis of...

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Bibliographic Details
Main Authors: Chirangini Pukhrambam, Irina O. Vvedenskaya, Bryce E. Nickels
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Molecular Biology
Gene Expression
Protein Biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005645

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http://www.sciencedirect.com/science/article/pii/S2666166721005645

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