Antigen Extraction and B Cell Activation Enable Identification of Rare Membrane Antigen Specific Human B Cells

Antigen Extraction and B Cell Activation Enable Identification of Rare Membrane Antigen Specific Human B Cells

Determining antigen specificity is vital for understanding B cell biology and for producing human monoclonal antibodies. We describe here a powerful method for identifying B cells that recognize membrane antigens expressed on cells. The technique depends on two characteristics of the interaction bet...

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Bibliographic Details
Main Authors: Maria Zimmermann, Natalie Rose, John M. Lindner, Hyein Kim, Ana Rita Gonçalves, Ilaria Callegari, Mohammedyaseen Syedbasha, Lukas Kaufmann, Adrian Egli, Raija L. P. Lindberg, Ludwig Kappos, Elisabetta Traggiai, Nicholas S. R. Sanderson, Tobias Derfuss
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-04-01
Series:Frontiers in Immunology
Subjects:
autoimmunity
membrane protein antigens
trogocytosis
human monoclonal antibodies
myasthenia gravis
Online Access:https://www.frontiersin.org/article/10.3389/fimmu.2019.00829/full

Internet

https://www.frontiersin.org/article/10.3389/fimmu.2019.00829/full

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