REQUIREMENTS FOR EFFICIENT PCR CLAMPING BY LOCKED NUCLEIC ACID OLIGONUCLEOTIES FOR SIMPLE AND SENSITIVE DETECTION OF SOMATIC MUTATIONS

REQUIREMENTS FOR EFFICIENT PCR CLAMPING BY LOCKED NUCLEIC ACID OLIGONUCLEOTIES FOR SIMPLE AND SENSITIVE DETECTION OF SOMATIC MUTATIONS

PCR clamping/wild-type blocking PCR with non-extendable locked nucleic acid (LNA) oligonucleotides is used for sensitive detection of somatic mutations in tumors. Various  versions of the technique use different DNA polymerases and LNA oligonucleotides with and  without additional phosphorothioate m...

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Bibliographic Details
Main Authors: V. A. Shamanin, I. V. Karpov, E. E. Pisareva, N. I. Gutkina, S. P. Kovalenko
Format: Article
Language:Russian
Published: Tomsk National Research Medical Center of the Russian Academy of Sciences 2018-09-01
Series:Sibirskij Onkologičeskij Žurnal
Subjects:
locked nucleic acid
pcr clamp
mutations
kras
braf
taq dna polymerase.
Online Access:https://www.siboncoj.ru/jour/article/view/812

Internet

https://www.siboncoj.ru/jour/article/view/812

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