Structure-guided engineering of adenine base editor with minimized RNA off-targeting activity

Base editors can induce transcriptome-wide RNA off-target editing independent of gRNA. Here, the authors engineer ABEmax variants with minimized RNA off-target activities.

Bibliographic Details
Main Authors:	Jianan Li, Wenxia Yu, Shisheng Huang, Susu Wu, Liping Li, Jiankui Zhou, Yu Cao, Xingxu Huang, Yunbo Qiao
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-04-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-021-22519-z

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spelling	doaj-4bd9786b378c4e9cb51a4b505bd699512021-04-18T11:13:57ZengNature Publishing GroupNature Communications2041-17232021-04-011211810.1038/s41467-021-22519-zStructure-guided engineering of adenine base editor with minimized RNA off-targeting activityJianan Li0Wenxia Yu1Shisheng Huang2Susu Wu3Liping Li4Jiankui Zhou5Yu Cao6Xingxu Huang7Yunbo Qiao8School of Life Science and Technology, ShanghaiTech UniversitySchool of Life Science and Technology, ShanghaiTech UniversitySchool of Life Science and Technology, ShanghaiTech UniversityPrecise Genome Engineering Center, School of Life Sciences, Guangzhou UniversityPrecise Genome Engineering Center, School of Life Sciences, Guangzhou UniversityPrecise Genome Engineering Center, School of Life Sciences, Guangzhou UniversityDepartment of Orthopaedics and Institute of Precision Medicine, Shanghai Key Laboratory of Orthopaedic Implant Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineSchool of Life Science and Technology, ShanghaiTech UniversityPrecise Genome Engineering Center, School of Life Sciences, Guangzhou UniversityBase editors can induce transcriptome-wide RNA off-target editing independent of gRNA. Here, the authors engineer ABEmax variants with minimized RNA off-target activities.https://doi.org/10.1038/s41467-021-22519-z
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Jianan Li Wenxia Yu Shisheng Huang Susu Wu Liping Li Jiankui Zhou Yu Cao Xingxu Huang Yunbo Qiao
spellingShingle	Jianan Li Wenxia Yu Shisheng Huang Susu Wu Liping Li Jiankui Zhou Yu Cao Xingxu Huang Yunbo Qiao Structure-guided engineering of adenine base editor with minimized RNA off-targeting activity Nature Communications
author_facet	Jianan Li Wenxia Yu Shisheng Huang Susu Wu Liping Li Jiankui Zhou Yu Cao Xingxu Huang Yunbo Qiao
author_sort	Jianan Li
title	Structure-guided engineering of adenine base editor with minimized RNA off-targeting activity
title_short	Structure-guided engineering of adenine base editor with minimized RNA off-targeting activity
title_full	Structure-guided engineering of adenine base editor with minimized RNA off-targeting activity
title_fullStr	Structure-guided engineering of adenine base editor with minimized RNA off-targeting activity
title_full_unstemmed	Structure-guided engineering of adenine base editor with minimized RNA off-targeting activity
title_sort	structure-guided engineering of adenine base editor with minimized rna off-targeting activity
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2021-04-01
description	Base editors can induce transcriptome-wide RNA off-target editing independent of gRNA. Here, the authors engineer ABEmax variants with minimized RNA off-target activities.
url	https://doi.org/10.1038/s41467-021-22519-z
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Structure-guided engineering of adenine base editor with minimized RNA off-targeting activity

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