A new and robust method of tethering IgG surrogate antigens on lipid bilayer membranes to facilitate the TIRFM based live cell and single molecule imaging experiments.

Our understanding of cell-cell interactions has been significantly improved in the past years with the help of Total Internal Reflection Fluorescence Microscope (TIRFM) in combination with an antigen presenting system supported by planar lipid bilayer (PLB) membranes, which are used to mimic the ext...

Full description

Bibliographic Details
Main Authors: Shaosen Zhang, Liling Xu, Xingwang Zhao, Xin Chen, Yilin Fan, Zhengpeng Wan, Yinsheng Xu, Wanli Liu
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3661735?pdf=render