Application of CRISPR/Cas9 lentiviral vector in construction of rat hepatic stellate cells with COX-2 gene knockout
ObjectiveTo obtain HSC-T6 cells with stable expression of Cas9 protein and HSC-T6-COX-2-/- cells with COX-2 gene defect by transfecting HSC-T6 cells with CRISPR/Cas9 lentiviral vector, and to provide a good method for further functional research and new strategies for the clinical treatment of liver...
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| Format: | Article |
| Language: | zho |
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Editorial Department of Journal of Clinical Hepatology
2021-02-01
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| Series: | Linchuang Gandanbing Zazhi |
| Online Access: | http://www.lcgdbzz.org/cn/article/doi/10.3969/j.issn.1001-5256.2021.02.018 |