Protocol for purification and identification of MHC class I immunopeptidome from cancer cell lines

Protocol for purification and identification of MHC class I immunopeptidome from cancer cell lines

Summary: Major histocompatibility complexes (MHC) play a critical role in immunity by presenting peptides on the cell surface for T cell recognition. Identification of these peptides can be valuable to develop vaccines or immunotherapeutic strategies for infectious diseases and cancers. Mass spectro...

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Main Authors: Sonali V. Mohan, Keshava K. Datta, Rebekah Ziegman, Corey Smith, Harsha Gowda
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Cancer
Immunology
Proteomics
Mass spectrometry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721000927
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spelling doaj-49fef723453a45dc803f47975d586f9f2021-03-22T12:53:35ZengElsevierSTAR Protocols2666-16672021-03-0121100385Protocol for purification and identification of MHC class I immunopeptidome from cancer cell linesSonali V. Mohan0Keshava K. Datta1Rebekah Ziegman2Corey Smith3Harsha Gowda4Cancer Precision Medicine Group, QIMR Berghofer Medical Research Institute, Herston, QLD, Australia; Faculty of Medicine, The University of Queensland, Herston, QLD, Australia; Corresponding authorCancer Precision Medicine Group, QIMR Berghofer Medical Research Institute, Herston, QLD, AustraliaCancer Precision Medicine Group, QIMR Berghofer Medical Research Institute, Herston, QLD, AustraliaSchool of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, QLD, Australia; QIMR Centre for Immunotherapy and Vaccine Development, QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia; Translational and Human Immunology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, QLD, AustraliaCancer Precision Medicine Group, QIMR Berghofer Medical Research Institute, Herston, QLD, Australia; Faculty of Medicine, The University of Queensland, Herston, QLD, Australia; School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, QLD, Australia; Corresponding authorSummary: Major histocompatibility complexes (MHC) play a critical role in immunity by presenting peptides on the cell surface for T cell recognition. Identification of these peptides can be valuable to develop vaccines or immunotherapeutic strategies for infectious diseases and cancers. Mass spectrometry is the only tool available for unbiased identification of the immunopeptidome. Here, we describe a protocol for purification and identification of MHC class I peptides, including in-house purification of anti-MHC-antibody from hybridoma cells and the LC-MS/MS analysis of MHC-I bound peptides.http://www.sciencedirect.com/science/article/pii/S2666166721000927CancerImmunologyProteomicsMass spectrometry
collection DOAJ
language English
format Article
sources DOAJ
author Sonali V. Mohan
Keshava K. Datta
Rebekah Ziegman
Corey Smith
Harsha Gowda
spellingShingle Sonali V. Mohan
Keshava K. Datta
Rebekah Ziegman
Corey Smith
Harsha Gowda
Protocol for purification and identification of MHC class I immunopeptidome from cancer cell lines
STAR Protocols
Cancer
Immunology
Proteomics
Mass spectrometry
author_facet Sonali V. Mohan
Keshava K. Datta
Rebekah Ziegman
Corey Smith
Harsha Gowda
author_sort Sonali V. Mohan
title Protocol for purification and identification of MHC class I immunopeptidome from cancer cell lines
title_short Protocol for purification and identification of MHC class I immunopeptidome from cancer cell lines
title_full Protocol for purification and identification of MHC class I immunopeptidome from cancer cell lines
title_fullStr Protocol for purification and identification of MHC class I immunopeptidome from cancer cell lines
title_full_unstemmed Protocol for purification and identification of MHC class I immunopeptidome from cancer cell lines
title_sort protocol for purification and identification of mhc class i immunopeptidome from cancer cell lines
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2021-03-01
description Summary: Major histocompatibility complexes (MHC) play a critical role in immunity by presenting peptides on the cell surface for T cell recognition. Identification of these peptides can be valuable to develop vaccines or immunotherapeutic strategies for infectious diseases and cancers. Mass spectrometry is the only tool available for unbiased identification of the immunopeptidome. Here, we describe a protocol for purification and identification of MHC class I peptides, including in-house purification of anti-MHC-antibody from hybridoma cells and the LC-MS/MS analysis of MHC-I bound peptides.
topic Cancer
Immunology
Proteomics
Mass spectrometry
url http://www.sciencedirect.com/science/article/pii/S2666166721000927
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