Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinica...

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Bibliographic Details
Main Authors: C.A.G. Leal, G.A. Carvalho-Castro, A.C. Cottorello, R.C. Leite, H.C.P. Figueiredo
Format: Article
Language:English
Published: Sociedade Brasileira de Microbiologia 2013-09-01
Series:Brazilian Journal of Microbiology
Subjects:
clinical samples
sensitivity
real-time PCR
WSSV
WSD
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300038&lng=en&tlng=en

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300038&lng=en&tlng=en

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