The critical role of DNA extraction for detection of mycobacteria in tissues.

BACKGROUND:Nucleic acid-based methods offer promise for both targeted and exploratory investigations of microbes in tissue samples. As the starting material for such studies is a mixture of host and microbial DNA, we have critically evaluated the DNA extraction step to determine the quantitative and...

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Bibliographic Details
Main Authors: Nicolas Radomski, Louis Kreitmann, Fiona McIntosh, Marcel A Behr
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3806855?pdf=render