High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy

The formation of cilia depends on centriole’s distal appendages. Here, the authors use correlative 3D-superresolution, electron microscopy and electron tomography to detail the architecture of distal appendages, to describe the initial stages of appendage assembly and show that appendages of mature...

Full description

Bibliographic Details
Main Authors:	Mathew Bowler, Dong Kong, Shufeng Sun, Rashmi Nanjundappa, Lauren Evans, Veronica Farmer, Andrew Holland, Moe R. Mahjoub, Haixin Sui, Jadranka Loncarek
Format:	Article
Language:	English
Published:	Nature Publishing Group 2019-03-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-018-08216-4

id	doaj-488ce455ae8b4d4ab0d73c6a0e625153
record_format	Article
spelling	doaj-488ce455ae8b4d4ab0d73c6a0e6251532021-05-11T12:23:11ZengNature Publishing GroupNature Communications2041-17232019-03-0110111510.1038/s41467-018-08216-4High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopyMathew Bowler0Dong Kong1Shufeng Sun2Rashmi Nanjundappa3Lauren Evans4Veronica Farmer5Andrew Holland6Moe R. Mahjoub7Haixin Sui8Jadranka Loncarek9Laboratory of Protein Dynamics and Signaling, NIH/NCI/CCRLaboratory of Protein Dynamics and Signaling, NIH/NCI/CCRWadsworth Center, New York State Department of HealthDepartment of Medicine (Nephrology Division), Washington UniversityDepartment of Molecular Biology & Genetics, Johns Hopkins University School of MedicineLaboratory of Protein Dynamics and Signaling, NIH/NCI/CCRDepartment of Molecular Biology & Genetics, Johns Hopkins University School of MedicineDepartment of Medicine (Nephrology Division), Washington UniversityWadsworth Center, New York State Department of HealthLaboratory of Protein Dynamics and Signaling, NIH/NCI/CCRThe formation of cilia depends on centriole’s distal appendages. Here, the authors use correlative 3D-superresolution, electron microscopy and electron tomography to detail the architecture of distal appendages, to describe the initial stages of appendage assembly and show that appendages of mature centrioles remodel during mitosis.https://doi.org/10.1038/s41467-018-08216-4
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Mathew Bowler Dong Kong Shufeng Sun Rashmi Nanjundappa Lauren Evans Veronica Farmer Andrew Holland Moe R. Mahjoub Haixin Sui Jadranka Loncarek
spellingShingle	Mathew Bowler Dong Kong Shufeng Sun Rashmi Nanjundappa Lauren Evans Veronica Farmer Andrew Holland Moe R. Mahjoub Haixin Sui Jadranka Loncarek High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy Nature Communications
author_facet	Mathew Bowler Dong Kong Shufeng Sun Rashmi Nanjundappa Lauren Evans Veronica Farmer Andrew Holland Moe R. Mahjoub Haixin Sui Jadranka Loncarek
author_sort	Mathew Bowler
title	High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy
title_short	High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy
title_full	High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy
title_fullStr	High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy
title_full_unstemmed	High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy
title_sort	high-resolution characterization of centriole distal appendage morphology and dynamics by correlative storm and electron microscopy
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2019-03-01
description	The formation of cilia depends on centriole’s distal appendages. Here, the authors use correlative 3D-superresolution, electron microscopy and electron tomography to detail the architecture of distal appendages, to describe the initial stages of appendage assembly and show that appendages of mature centrioles remodel during mitosis.
url	https://doi.org/10.1038/s41467-018-08216-4
work_keys_str_mv	AT mathewbowler highresolutioncharacterizationofcentrioledistalappendagemorphologyanddynamicsbycorrelativestormandelectronmicroscopy AT dongkong highresolutioncharacterizationofcentrioledistalappendagemorphologyanddynamicsbycorrelativestormandelectronmicroscopy AT shufengsun highresolutioncharacterizationofcentrioledistalappendagemorphologyanddynamicsbycorrelativestormandelectronmicroscopy AT rashminanjundappa highresolutioncharacterizationofcentrioledistalappendagemorphologyanddynamicsbycorrelativestormandelectronmicroscopy AT laurenevans highresolutioncharacterizationofcentrioledistalappendagemorphologyanddynamicsbycorrelativestormandelectronmicroscopy AT veronicafarmer highresolutioncharacterizationofcentrioledistalappendagemorphologyanddynamicsbycorrelativestormandelectronmicroscopy AT andrewholland highresolutioncharacterizationofcentrioledistalappendagemorphologyanddynamicsbycorrelativestormandelectronmicroscopy AT moermahjoub highresolutioncharacterizationofcentrioledistalappendagemorphologyanddynamicsbycorrelativestormandelectronmicroscopy AT haixinsui highresolutioncharacterizationofcentrioledistalappendagemorphologyanddynamicsbycorrelativestormandelectronmicroscopy AT jadrankaloncarek highresolutioncharacterizationofcentrioledistalappendagemorphologyanddynamicsbycorrelativestormandelectronmicroscopy
_version_	1721444912730210304

High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy

Similar Items