A set of novel CRISPR-based integrative vectors for Saccharomyces cerevisiae [version 1; referees: 2 approved]
Integrating a desired DNA sequence into yeast genomes is a widely-used genetic manipulation in the budding yeast Saccharomyces cerevisiae. The conventional integration method is to use an integrative plasmid such as pRS or YIplac series as the target DNA carrier. The nature of this method risks mult...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Wellcome
2018-06-01
|
Series: | Wellcome Open Research |
Online Access: | https://wellcomeopenresearch.org/articles/3-72/v1 |