A Simple Cloning-free Method to Efficiently Induce Gene Expression Using CRISPR/Cas9

A Simple Cloning-free Method to Efficiently Induce Gene Expression Using CRISPR/Cas9

Gain-of-function studies often require the tedious cloning of transgene cDNA into vectors for overexpression beyond the physiological expression levels. The rapid development of CRISPR/Cas technology presents promising opportunities to address these issues. Here, we report a simple, cloning-free met...

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Bibliographic Details
Main Authors: Lyujie Fang, Sandy S.C. Hung, Jennifer Yek, Layal El Wazan, Tu Nguyen, Shahnaz Khan, Shiang Y. Lim, Alex W. Hewitt, Raymond C.B. Wong
Format: Article
Language:English
Published: Elsevier 2019-03-01
Series:Molecular Therapy: Nucleic Acids
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253118303020

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http://www.sciencedirect.com/science/article/pii/S2162253118303020

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