Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel

Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel

SthK, a cyclic nucleotide-gated ion channel from Spirochaeta thermophila activates slowly upon cAMP increase. Here, authors investigate cAMP-induced activation in purified SthK channels using stopped-flow assays and enzymatic catalysis and reveal that the cis/trans conformation of a conserved prolin...

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Main Authors: Philipp A. M. Schmidpeter, Jan Rheinberger, Crina M. Nimigean
Format: Article
Language:English
Published: Nature Publishing Group 2020-12-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-20104-4
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spelling doaj-472b03d2ec91449c83984880c8a210032021-01-31T13:36:37ZengNature Publishing GroupNature Communications2041-17232020-12-0111111210.1038/s41467-020-20104-4Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channelPhilipp A. M. Schmidpeter0Jan Rheinberger1Crina M. Nimigean2Weill Cornell Medicine, Department of AnesthesiologyWeill Cornell Medicine, Department of AnesthesiologyWeill Cornell Medicine, Department of AnesthesiologySthK, a cyclic nucleotide-gated ion channel from Spirochaeta thermophila activates slowly upon cAMP increase. Here, authors investigate cAMP-induced activation in purified SthK channels using stopped-flow assays and enzymatic catalysis and reveal that the cis/trans conformation of a conserved proline in the cyclic nucleotide-binding domain determines the activation kinetics of SthK.https://doi.org/10.1038/s41467-020-20104-4
collection DOAJ
language English
format Article
sources DOAJ
author Philipp A. M. Schmidpeter
Jan Rheinberger
Crina M. Nimigean
spellingShingle Philipp A. M. Schmidpeter
Jan Rheinberger
Crina M. Nimigean
Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel
Nature Communications
author_facet Philipp A. M. Schmidpeter
Jan Rheinberger
Crina M. Nimigean
author_sort Philipp A. M. Schmidpeter
title Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel
title_short Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel
title_full Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel
title_fullStr Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel
title_full_unstemmed Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel
title_sort prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2020-12-01
description SthK, a cyclic nucleotide-gated ion channel from Spirochaeta thermophila activates slowly upon cAMP increase. Here, authors investigate cAMP-induced activation in purified SthK channels using stopped-flow assays and enzymatic catalysis and reveal that the cis/trans conformation of a conserved proline in the cyclic nucleotide-binding domain determines the activation kinetics of SthK.
url https://doi.org/10.1038/s41467-020-20104-4
work_keys_str_mv AT philippamschmidpeter prolylisomerizationcontrolsactivationkineticsofacyclicnucleotidegatedionchannel
AT janrheinberger prolylisomerizationcontrolsactivationkineticsofacyclicnucleotidegatedionchannel
AT crinamnimigean prolylisomerizationcontrolsactivationkineticsofacyclicnucleotidegatedionchannel
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