Standard protocol for the total red blood cell Pig-a assay used in the interlaboratory trial organized by the Mammalian Mutagenicity Study Group of the Japanese Environmental Mutagen Society

Standard protocol for the total red blood cell Pig-a assay used in the interlaboratory trial organized by the Mammalian Mutagenicity Study Group of the Japanese Environmental Mutagen Society

Abstract The Pig-a assay, a promising tool for evaluating in vivo genotoxicity, is based on flow cytometric enumeration of red blood cells (RBCs) that are deficient in glycosylphosphatidylinositol anchor protein. Various approaches for measuring Pig-a mutant cells have been developed, particularly f...

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Bibliographic Details
Main Authors: Satsuki Chikura, Takafumi Kimoto, Satoru Itoh, Hisakazu Sanada, Shigeharu Muto, Katsuyoshi Horibata
Format: Article
Language:English
Published: BMC 2019-02-01
Series:Genes and Environment
Subjects:
Pig-a assay
Glycosylphosphatidylinositol
Flow cytometry
Red blood cells
In vivo gene mutation
CD59
Online Access:http://link.springer.com/article/10.1186/s41021-019-0121-z

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http://link.springer.com/article/10.1186/s41021-019-0121-z

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