Centrifugal Microfluidic Integration of 4-Plex ddPCR Demonstrated by the Quantification of Cancer-Associated Point Mutations

Centrifugal Microfluidic Integration of 4-Plex ddPCR Demonstrated by the Quantification of Cancer-Associated Point Mutations

We present the centrifugal microfluidic implementation of a four-plex digital droplet polymerase chain reaction (ddPCR). The platform features 12 identical ddPCR units on a LabDisk cartridge, each capable of generating droplets with a diameter of 82.7 ± 9 µm. By investigating different oil–surfactan...

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Bibliographic Details
Main Authors: Franziska Schlenker, Elena Kipf, Nadine Borst, Nils Paust, Roland Zengerle, Felix von Stetten, Peter Juelg, Tobias Hutzenlaub
Format: Article
Language:English
Published: MDPI AG 2021-01-01
Series:Processes
Subjects:
microfluidics
digital droplet polymerase chain reaction (ddPCR)
multiplexing
centrifugal step emulsification
droplet stability
droplet fluorescence evaluation
Online Access:https://www.mdpi.com/2227-9717/9/1/97

Internet

https://www.mdpi.com/2227-9717/9/1/97

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