Purification and characterization of highly stable aldo-keto reductase from camel (Camelus dromedarius) liver

Purification and characterization of highly stable aldo-keto reductase from camel (Camelus dromedarius) liver

Aldo-keto reductase (AKR) was purified to homogeneity from camel liver by ion exchange on Q Sepharose, affinity chromatography on Blue-Sepharose and 2,5-ADP-Sepharose 4B. The purification procedure resulted in 32.43-fold purification with 0.65% final yield. Subunit and native molecular weights of...

Full description

Bibliographic Details
Main Authors: Al-Harbi Ibrahim A., Al-Senaidy Abdurrahman M., Ismael Mohammad A.
Format: Article
Language:English
Published: University of Belgrade, University of Novi Sad 2013-01-01
Series:Archives of Biological Sciences
Subjects:
Camel
purification
aldo-keto reductase
NAD(P)H
characterization
m-Nitrobenzaldehyde
4-Anisaldehyde
P-Benzoquinone
Online Access:http://www.doiserbia.nb.rs/img/doi/0354-4664/2013/0354-46641301113A.pdf

Internet

http://www.doiserbia.nb.rs/img/doi/0354-4664/2013/0354-46641301113A.pdf

Similar Items