A Model System for Concurrent Detection of Antigen and Antibody Based on Immunological Fluorescent Method

This paper describes a combined antigen/antibody immunoassay implemented in a 96-well plate using fluorescent spectroscopic method. First, goat anti-human IgG was used to capture human IgG (model antigen); goat anti-human IgG (Cy3 or FITC) was used to detect the model antigen; a saturating level of...

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Bibliographic Details
Main Author: Yuan-Cheng Cao
Format: Article
Language:English
Published: Hindawi Limited 2015-01-01
Series:Journal of Spectroscopy
Online Access:http://dx.doi.org/10.1155/2015/248504