Cholesterol-Dependent Energy Transfer between Fluorescent Proteins—Insights into Protein Proximity of APP and BACE1 in Different Membranes in Niemann-Pick Type C Disease Cells

Cholesterol-Dependent Energy Transfer between Fluorescent Proteins—Insights into Protein Proximity of APP and BACE1 in Different Membranes in Niemann-Pick Type C Disease Cells

Förster resonance energy transfer (FRET) -based techniques have recently been applied to study the interactions between β-site APP-cleaving enzyme-GFP (BACE1-GFP) and amyloid precursor protein-mRFP (APP-mRFP) in U373 glioblastoma cells. In this context, the role of APP-BACE...

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Bibliographic Details
Main Authors: Bjoern von Einem, Petra Weber, Michael Wagner, Martina Malnar, Marko Kosicek, Silva Hecimovic, Christine A. F. von Arnim, Herbert Schneckenburger
Format: Article
Language:English
Published: MDPI AG 2012-11-01
Series:International Journal of Molecular Sciences
Subjects:
FRET
cholesterol
APP
BACE1
NPC
TIRFM
neurodegeneration
laurdan
generalized polarization
Online Access:http://www.mdpi.com/1422-0067/13/12/15801

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http://www.mdpi.com/1422-0067/13/12/15801

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