Simple and fast quantification of DNA damage by real-time PCR, and its application to nuclear and mitochondrial DNA from multiple tissues of aging zebrafish

Simple and fast quantification of DNA damage by real-time PCR, and its application to nuclear and mitochondrial DNA from multiple tissues of aging zebrafish

Abstract We describe a real-time (rt) PCR-based method of quantifying DNA damage, adapted from the long-run rtPCR method of DNA damage quantification (LORD-Q) developed by Lehle et al. (Nucleic Acids Res 42(6):e41, 2014). We show that semi-long run rtPCR, which generates amplicons half the length of...

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Bibliographic Details
Main Authors: Shusen Zhu, James A. Coffman
Format: Article
Language:English
Published: BMC 2017-07-01
Series:BMC Research Notes
Subjects:
DNA damage
Quantitative PCR
Nuclear
Mitochondrial
Aging
Zebrafish
Online Access:http://link.springer.com/article/10.1186/s13104-017-2593-x

Internet

http://link.springer.com/article/10.1186/s13104-017-2593-x

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